Advanced Diagnostic Testing for Immune and Inflammatory Conditions
( CIRS )

Comprehensive Testing for Precision Health Monitoring and Personalized Care

HumanBiomics

Test Details

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Human TGF-beta 1 ELISA

TGF-beta 1 is crucial for tissue regeneration, cell differentiation, embryonic development, and immune modulation. It is found in hematopoietic tissue and activates a pathway that inhibits early cancer cell progression.
TGF-beta 1 aids in extracellular matrix deposition, wound healing, and cirrhosis development. It is produced by various cells, and most have specific receptors for it.

Preferred Specimen(s)

1 mL platelet-free plasma collected in EDTA (lavender-top) tube

Minimum Volume

0.25 mL

Collection Instructions

PSC Collections: Collect in EDTA (lavender-top) tube and immediately centrifuge to prepare platelet-poor plasma. Decant the plasma into another pour-over tube. Centrifuge a second time and pour over plasma. Freeze immediately.
Client Collections: Collect in EDTA (lavender-top) tube. Centrifuge for 15 minutes at 1000 X g within 30 minutes of collection. Centrifuge plasma again at 3000 X g for 10 minutes for complete platelet removal. Freeze Immediately.
For fixed speed centrifuges such as 645e: Collect in EDTA (lavender-top) tube. Centrifuge 3 times for 10 minutes at 1600 X g while decanting the plasma each time before the next spin within 30 minutes of collection. Freeze immediately.

Transport Container

Transport tube
Freeze plasma or serum before shipping

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

Room temperature: Not established
Refrigerated: 48 hours
Frozen -20° C: 14 days
Frozen -70° C: 30 days

Reject Criteria

Hemolysis • Grossly lipemic • Specimens with particulate matter or microbial contamination • Specimens outside of listed stability

Human MSH/POMC ELISA

Alpha MSH is a 13-amino acid peptide (1665 kDa) derived from pro-opiomelanocorticotropin, sharing its sequence with ACTH 1-13. It stimulates melanin production and melanosome dispersion in skin melanocytes, and also promotes aldosterone synthesis.

Plasma alpha MSH levels increase during endotoxin-induced fever and are elevated in insulin-resistant obese men and AIDS patients.

Preferred Specimen(s)

3 mL frozen plasma collected in an EDTA (lavender-top) tube

Minimum Volume

1 mL

Transport Container

Transport tube
Freeze plasma or serum before shipping

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 90 days

Reject Criteria

Hemolysis • Grossly lipemic • Specimens with particulate matter or microbial contamination • Specimens outside of listed stability

Human VEGF ELISA

The Vascular Endothelial Growth Factor (VEGF) ELISA is designated solely for research purposes. It may hold significance in assessing patients with different cancer types who are candidates for anti-angiogenesis therapy.

Preferred Specimen(s)

1 mL plasma collected in an EDTA (lavender-top) tube

Minimum Volume

0.3 mL

Collection Instructions

Mix the sample and centrifuge immediately after collection to separate plasma from cells. Transfer plasma to a plastic specimen transport container and mark the specimen type as plasma on the container. Freeze immediately. Cytokine levels may demonstrate diurnal variation. Recommend cytokine levels be determined at the same time of day for improved longitudinal comparison.

Transport Container

Transport tube
Freeze plasma or serum before shipping

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

Room temperature: 4 hours
Refrigerated: 48
hours Frozen: 1 year

Reject Criteria

Hemolysis • Grossly lipemic • Specimens with particulate matter or microbial contamination • Specimens outside of listed stability

Human MMP 9-ELISA ( Serum )

MMP participates in inflammation, tissue remodeling, wound healing, and cytokine processing. The expression of MMP-9 is associated with aberrant collagen deposition in pancreatic cancer, as well as the metastasis to lymph nodes by human breast cancer cells and the invasion of regional arteries in giant cell tumors of bone.

Preferred Specimen(s)

1 mL serum

Minimum Volume

0.5 mL

Specimen Container

Plastic screw-cap vial
Freeze plasma or serum before shipping

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

Room temperature: 4 hours
Refrigerated: 24 hours
Frozen: 30 days

Reject Criteria

Hemolysis • Grossly lipemic • Specimens with particulate matter or microbial contamination • Specimens outside of listed stability

Human Complement C4a ELISA

Reduced levels of Complement Component C4c are linked to acute systemic lupus erythematosus, glomerulonephritis, immune complex disorders, cryoglobulinemia, congenital C4 deficiency, and generalized autoimmune diseases.

Preferred Specimen(s)

1 mL serum

Minimum Volume

0.5 mL

Transport Container

Transport tube
Freeze plasma or serum before shipping

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

Room temperature: Unacceptable
Refrigerated: 4 days
Frozen: 21 days

Reject Criteria

Hemolysis • Grossly lipemic • Specimens with particulate matter or microbial contamination • Specimens outside of listed stability

Why Consider These Tests?

These advanced diagnostic tests are crucial for individuals seeking to:

Monitor Immune Health: Detect and track immune system function, helping to identify imbalances that may lead to chronic conditions.
Address Inflammatory Issues: Pinpoint the root causes of inflammation in the body, aiding in early diagnosis and more effective treatment plans for various inflammatory diseases.
Detect Genetic Predispositions: Identify genetic markers that could influence how your body responds to environmental factors, infections, or allergens.
Support Chronic Illness Management: These tests are valuable tools for patients suffering from long-term conditions like autoimmune diseases, helping healthcare providers tailor treatments.
Guide Personalized Therapies: By understanding specific biomarkers like TGF-β1 and VEGF, your healthcare team can create more personalized and targeted interventions to improve health outcomes.