Endotoxin Testing (Kinetic LAL Assay)
Endotoxin sample collection methods.
1. Dust samples: Collect sample using a Swiffer cloth or Vacuum Cone with the kit.
2. Air samples: Collect sample using Air Cassette provided with the kit.
Keep the cassette and vacuum cone in a cool dry place, sealed in its protective sleeve. Return the exposed cassette or cone to the laboratory providing the analysis within the shortest possible time, preferably within <24 hours since exposure.
The vacuum cone and cassette are provided sterile and can also be used to sample for Glucans.
Endotoxins are derived from the cell walls of Gram-Negative bacteria (GNB). Gram-negative bacteria are found almost anywhere in nature. Their toxins are found within the outer layer membrane of the GNB cell wall. They do not have to be living, therefore both viable and non-viable gram-negative bacteria contribute endotoxins.
The most common routes of endotoxin exposure are through inhalation, or through intestinal tract absorption through food or water. The health effect of endotoxins varies depending on the individual, dosage, and route of exposure. Elevated airborne concentrations are prevalent in sewage treatment plants, swine operations, cotton textile mills, poultry houses and in water damaged and flood contaminated buildings.